Control of DNA integrity
Continued cooperation with the Institute of Animal Biology and Genetics, Libechov (Czech Republic) was focused on the study of processes controlling the integrity of the DNA molecule. Published results have demonstrated that during maturation of female gametes MRE11 nuclear proteins is more important in the recognition of DNA damage when compared with ATM kinase. It was also confirmed that this endonuclear protein (MRE11) is essential for the subsequent maintenance of the chromosomes integrity during all maturing stages of oocyte.
Mayer A, Baran V, Sakakibara Y, Brzakova A, Ferencova I, Motlik J, Kitajima TS, Schultz RM, Solc S. DNA damage response during mouse oocyte maturation. Cell Cycle 25(4) 2016, 546-558. (3.952 – IF2015), (2016 – Current Contents), ISSN 1538-4101. https://www.ncbi.nlm.nih.gov/pubmed/26745237
Manganese in animal nutrition
Intensive breeding focuses on the increasing animal production results in the higher mineral levels in feed than recommended requirements. Better Mn bioavailability from its organic dietary sources could increase mineral tissue deposition in animals. The 4-month supplementation of lamb diet with Mn chelate of glycine hydrate as an organic Mn source or with inorganic manganese sulphate (MnSO4) with a total Mn content allowed in the EU up to 150 mg/kg increased the Mn levels in lamb plasma and liver. The MnSOD and arginase activity was increased in liver tissue too. These parameters reflect the Mn dietary intake, regardless of the Mn dietary source. Higher Mn concentration in kidney cortex of lambs fed a diet enriched with the organic Mn source could suggest some differences in the mineral distribution between Mn dietary sources. The presented results did not indicate any differences between dietary Mn sources either in mineral tissue deposition (Mn, Zn, Cu) or activity of MnSOD and arginase in lamb tissues. However, it seems that manganese intake of up to 150 mg/kg in feed may decrease plasma Cu concentration in lambs due to the increased Cu accumulation in liver tissue.
GREŠÁKOVÁ, Ľubomíra- VENGLOVSKÁ, Katarína- ČOBANOVÁ, Klaudia. Dietary manganese source does not affect Mn, Zn and Cu tissue deposition and the activity of manganese-containing enzymes in lambs. In Journal of Trace Elements in Medicine and Biology, 2016, vol.38, p. 138-143. (2.55- IF2015). (2016 – Current Contents). ISSN 0946-672X. http://dx.doi.org/10.1016/j.jtemb.2016.05.003
New species of bacteria from extreme environment of brown mud disposal site near Žiar nad Hronom
Brown mud is a waste by-product of alumina production by Bayer process. Due to extensive sodium hydroxide use in the process, brown mud disposal site near Ziar nad Hronom (Banska Bystrica region, Slovakia) and drainage water are ones of the greatest environmental burdens in Slovakia. Drainage water from this landfills has pH value higher than 13, and it contains many heavy metals and elevated salt content. Despite extreme conditions relatively numerous bacterial population was detected in the drainage water with frequency of about 80 cfu/ml using cultivation approach. The alkalitolerant heterotrophic isolates were identified by combination of MALDI-TOF and 16S rDNA analysis. Drainage water population was dominated by Actinobacteria (Microbacterium spp. and Micrococcus spp.) followed by low G + C-content gram-positive bacteria (Bacillus spp.). Two isolates belonged to gram-negative bacteria only, identified as Brevundimonas spp. Phylogenetic and biochemical analyses indicate that nearly half of the bacteria isolated are probably representatives of a new species. Brown mud disposal site is proposed as a source of new bacterial taxa possibly used in biotechnology and bioremediation processes.
STRAMOVÁ, Zuzana – REMENÁR, Matej – JAVORSKÝ, Peter – PRISTAŠ, Peter. Heterotrophic microflora of highly alkaline (pH13) brown mud disposal site drainage water near Ziar nad Hronom (Banska Bystrica region, Slovakia). In Environmental science and pollution research, 2016, vol. 23, no. 5, p. 4199-4206. (2.760 – IF2015). (2016 – Current Contents). ISSN 0944-1344.(ITMS 26220120001. http://link.springer.com/article/10.1007/s11356-015-4842-7