Research in Laboratory of the Animal Physiology and Metabolism is focused on the bioavailability of trace elements (Zn, Mn, Se) from their various, prevalently organic sources that are currently used as feed additives in nutrition of farm animals. The principal objective of our study is also to estimate the optimal content of trace elements in feed for current modern breeds of farm animals without negative effects on their health and performance what would result in a substantial reduction of environmental contamination by zinc from animal husbandry. In term of consumer safety, our results showed that the use of organic manganese chelates in animal nutrition does not raise any safety concern for consumers provided that EU total maximum authorised level of manganese in complete feed is respected. The response of animal antioxidant status to various sources and doses of microelements in diet is evaluated by the measurement of antioxidant enzyme activities in tissues.
prof. DVM. Štefan Faix, DSc., RNDr. Klaudia Čobanová, PhD., DVM. Ľubomíra Grešáková, PhD., DVM. Ľubomír Leng, DSc., DVM. Iveta Plachá, PhD.
- Atomic Absorption Spectrophotometer (AAS) for quantitative analysis of macro- and microelements.
- Multi-mode Microplate Reader Synergy 2 (Biotek) for absorbance, fluorescence and luminiscence measurements in microplates.
- UV VIS Spectrophotometer (Shimadzu) for determination of antioxidant enzyme activities.
- Fluorescence Spectrometer Lumina (Thermo Scientific) for measurement of standard biochemical parameters.
- Micro Ultracentrifuge (Hitachi) for separation of cellular components/organelles.